英文名称:
Human α1ACT(Alpha 1 Antichymotrypsin) ELISA Kit
别称:
SERPINA3, AACT, ACT, GIG24, GIG25, serpin family A member 3
样品类型:
Serum, Plasma, Tissue homogenate and Other biological samples
研究领域:
Apoptosis;Tumor immunity;Infection immunity;Immune molecule;
测试原理:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to α1ACT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to α1ACT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain α1ACT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of α1ACT in the samples is then determined by comparing the OD of the samples to the standard curve.