英文名称:
Human eIF3a(Eukaryotic Translation Initiation Factor 3a) ELISA Kit
别称:
EIF3A, EIF3, EIF3S1, P167, TIF32, eIF3-p17, eIF3-theta, p18, p185, eukaryotic translation initiation factor 3 subunit A
样品类型:
Serum, Plasma, Tissue homogenate and Other biological samples
研究领域:
Metabolic pathway;Endocrinology;Hormone metabolism;
测试原理:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to eIF3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to eIF3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain eIF3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of eIF3a in the samples is then determined by comparing the OD of the samples to the standard curve.