别称:
Phospholipase C Beta 1,Phospholipase C, Beta 1 (Phosphoinositide-Specific),Phospholipase C-I,EC 3.1.4.11,PLC-Beta-1,PLC-154,PLC-I,1-Phosphatidylinositol 4,5-Bisphosphate Phosphodiesterase Beta-1,1-Phosphatidyl-D-Myo-Inositol-4,5-Bisphosphate,Monophosphatidylinositol Phosphodiesterase,Phosphoinositide Phospholipase C-Beta-1,Triphosphoinositide Phosphodiesterase,Inositoltrisphosphohydrolase,Phospholipase C-Beta-1,Phosphoinositidase C,KIAA581,PI-PLC,EIEE12,PLC154,PLCB1A,PLCB1B
测试原理:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLCβ1/PI-PLC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PLCβ1/PI-PLC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLCβ1/PI-PLC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PLCβ1/PI-PLC in the samples is then determined by comparing the OD of the samples to the standard curve.