Human NLRP3(NLR Family, Pyrin Domain Containing Protein 3) ELISA Kit

Human

AGTAVPRL ; AII/AVP ; Angiotensin/vasopressin receptor AII/AVP like ; Angiotensin/vasopressin receptor AII/AVP-like ; C1orf7 ; Caterpiller protein 1.1 ; CIAS 1 ; CIAS1 ; CLR1.1 ; Cold autoinflammatory syndrome 1 ; Cold autoinflammatory syndrome 1 protein ; Cryopyrin ; Familial cold autoinflammatory syndrome ; FCAS ; FCU ; LRR and PYD domains-containing protein 3 ; Muckle-Wells syndrome ; MWS ; NACHT ; NACHT LRR and PYD containing protein 3 ; NALP 3 ; NALP3 ; NALP3 ; NLR family pyrin domain containing 3 ; NLRP3 ; PYPAF 1 ; PYPAF1 ; PYRIN containing APAF1 like protein 1 ; PYRIN-containing APAF1-like protein 1

Q96P20

Sandwich

10ng/mL

0.1ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：100μL

Cancer, Immunology;

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NLRP3 .Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NLRP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NLRP3 ,biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NLRP3 You can calculate the concentration of Human NLRP3 in the samples by comparing the OD of the samples to the standard curve.