英文名称:
Rat PECAM1/CD31(Platelet/Endothelial Cell Adhesion Molecule 1) ELISA Kit
别称:
Platelet endothelial cell adhesion molecule,PECAM-1,cluster of differentiation 31,CD31,CD31/EndoCAM, GPIIA', PECA1, PECAM-1, endoCAM
样品类型:
Serum, Plasma, Tissue homogenate and Other biological samples
研究领域:
Signal transduction;Metabolic pathway;Neuro science;Hormone metabolism;
测试原理:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PECAM1/CD31. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PECAM1/CD31. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PECAM1/CD31, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PECAM1/CD31 in the samples is then determined by comparing the OD of the samples to the standard curve.