英文名称:
Rat HSPA4(Heat Shock 70kDa Protein 4) ELISA Kit
别称:
HSPA4, APG-2, HEL-S-5a, HS24/P52, HSPH2, RY, hsp7, hsp7RY, heat shock protein family A (Hsp7) member 4
样品类型:
Serum, Plasma, Tissue homogenate and Other biological samples
测试原理:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSPA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HSPA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSPA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HSPA4 in the samples is then determined by comparing the OD of the samples to the standard curve.