英文名称:
Mouse SSRP1(Structure Specific Recognition Protein 1) ELISA Kit
别称:
FACT complex subunit SSRP1,FACT, FACT8, T16,Chromatin-specific transcription elongation factor 8 kDa subunit,Facilitates chromatin transcription complex 8 kDa subunit,FACT 8 kDa subunit,FACTp8,Facilitates chromatin transcription complex subunit SSRP1,Recombination signal sequence recognition protein 1,hSSRP1
样品类型:
Serum, Plasma, Tissue homogenate and Other biological samples
研究领域:
Signal transduction;
测试原理:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SSRP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SSRP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SSRP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SSRP1 in the samples is then determined by comparing the OD of the samples to the standard curve.