英文名称:
Mouse HSP-70/HSPA9(Heat Shock Protein 70) ELISA Kit
别称:
HSPA9, CSA, GRP-75, GRP75, HSPA9B, MOT, MOT2, MTHSP75, PBP74
Uniport ID:
Q61696&P17879
样品类型:
Serum, Plasma, Tissue homogenate and Other biological samples
研究领域:
Endocrinology;Pulmonology;Hepatology;Hormone metabolism;
测试原理:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP-70/HSPA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HSP-70/HSPA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSP-70/HSPA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HSP-70/HSPA9 in the samples is then determined by comparing the OD of the samples to the standard curve.