通用型ELISA试剂盒
EETs(Epoxyeicosatrienoic acid)ELISA Kit
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货号
FY-EU5891样本类型
Serum, Plasma and Other biological fluids检测时间
1.5H采购详情
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产品详情
英文名称:
EETs(Epoxyeicosatrienoic acid)ELISA Kit
种属:
Universal
别称:
Epoxyeicosatrienoic acid-d11、(±)11(12)-EET-d11
检测方法:
Competitive
标准品:
10ng/mL
灵敏度:
0.09 ng/mL
检测范围:
0.16-10ng/mL
反应时间:
1.5H
样品类型:
Serum, Plasma and Other biological fluids
技术数据
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
| Concentration | OD1 | OD2 | Average OD | Corrected |
|---|---|---|---|---|
| 10 | 0.216 | |||
| 5 | 0.407 | |||
| 2.5 | 0.693 | |||
| 1.25 | 0.974 | |||
| 0.63 | 1.249 | |||
| 0.31 | 1.475 | |||
| 0.16 | 1.943 | |||
| 1 | 2.436 |
线性
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 91-98 | 97-106 | 93-99 |
| Average (%) | 99 | 93 | 99 | |
| 1:4 | Range (%) | 92-99 | 89-98 | 92-100 |
| Average (%) | 95 | 96 | 98 | |
| 1:8 | Range (%) | 94-102 | 89-97 | 95-103 |
| Average (%) | 93 | 93 | 97 | |
| 1:16 | Range (%) | 88-98 | 96-104 | 97-104 |
| Average (%) | 97 | 100 | 93 |
精密度
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
| Item | Intra-assay Precision | Intra-assay Precision |
|---|---|---|
| Sample | 3 | 3 |
| Replicate | 9 | 18 |
| CV(%) | 5 | 8 |
回收率
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range(%) | Average Recovery(%) |
|---|---|---|
| Serum (n=8) | 88-96 | 92 |
| EDTA plasma (n=8) | 91-100 | 97 |
| Cell culture media (n=8) | 90-100 | 94 |
检测流程图
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