EasyPro Mouse GC(Glucagon) ELISA Kit

Mouse

Carbohydrate-Rich Portion of Platelet Membrane Glycoprotein Ib Alpha Polypeptide

P55095

Competitive

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Metabolic pathway;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GC Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for GC are added to the micro ELISA plate wells. GC in samples (or standards) competes with a fixed amount of GC on the solid phase supporter for sites on the HRP linked detection antibody specific to GC. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of GC in the samples is then determined by comparing the OD of the samples to the standard curve.