Product Name in English:
Human F3/TF(Tissue Factor) ELISA Kit
Alternative Name:
F3, CD142, TF, TFA, coagulation factor III, tissue factor
Detection Range:
7.82-500pg/mL
Sample Type:
Serum, Plasma, Tissue homogenate and Other biological samples
Research:
Cytokine;Tumor immunity;Infection immunity;Immune molecule;Hematology;
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to F3/TF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to F3/TF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F3/TF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F3/TF in the samples is then determined by comparing the OD of the samples to the standard curve.