Product Name in English:
Human CCT6A(T-complex 1 protein subunit zeta) ELISA Kit
Alternative Name:
CCT6A, CCT-zeta, CCT-zeta-1, CCT6, Cctz, HTR3, MoDP-2, TCP-1-zeta, TCP2, TCPZ, TTCP2
Detection Range:
31.25-2000pg/mL
Sample Type:
Serum, Plasma, Tissue homogenate and Other biological samples
Research:
Metabolic pathway;Neuro science;Developmental science;
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCT6A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CCT6A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CCT6A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CCT6A in the samples is then determined by comparing the OD of the samples to the standard curve.