Product Name in English:
Human MRC2(Mannose Receptor C Type 2) ELISA Kit
Alternative Name:
CD28,C-type lectin domain family 13 member E,Endocytic receptor 18,Macrophage mannose receptor 2,Urokinase-type plasminogen activator receptor-associated protein,UPAR-associated protein,Urokinase receptor-associated protein
Detection Range:
0.32-20ng/mL
Sample Type:
Serum, Plasma, Tissue homogenate and Other biological samples
Research:
Metabolic pathway;Endocrinology;Cardiovascular biology;Developmental science;Bone metabolism;
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MRC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MRC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MRC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MRC2 in the samples is then determined by comparing the OD of the samples to the standard curve.