Product Name in English:
Mouse sMHC-2(soluble myosin heavy chain 2) ELISA Kit
Alternative Name:
Myosin-2, MYH2, Myosin heavy chain 2, Myosin heavy chain 2a, MyHC-2a, Myosin heavy chain IIa, MyHC-IIa, Myosin heavy chain, skeletal muscle, adult 2, MYHSA2
Detection Range:
0.32-20ng/mL
Sample Type:
Serum, Plasma, Tissue homogenate and Other biological samples
Research:
Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to sMHC-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to sMHC-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain sMHC-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of sMHC-2 in the samples is then determined by comparing the OD of the samples to the standard curve.