cGMP(Cyclic Guanosine Monophosphate) ELISA Kit

Universal

c-GMP; 3',5'-Cyclic GMP; Cyclic 3',5'-GMP; Guanosine 3',5'-Cyclic Phosphate

Competitive

50 pmol/mL

0.47 pmol/mL

0.78-50 pmol/mL

1.5H

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids

Signal transduction;Metabolic pathway;

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with cGMP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to cGMP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cGMP in the samples is then determined by comparing the OD of the samples to the standard curve.