NE(Noradrenaline) ELISA Kit

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Product No

FY-EU1161

Sample Type

Serum, Plasma, Cell lysates, Cell culture supernates and Other biological fluids

Assay Time

1.5H

Price($)

280 400

Size

48T 96T

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Product Details

Product Name in English:

NE(Noradrenaline) ELISA Kit

Species:

Universal

Alternative Name:

NA; NAd; Norepinephrine; Norepi

Assay Type:

Competitive

Standard:

5000 pg/mL

Sensitivity:

26.1 pg/mL

Detection Range:

78.13-5000 pg/mL

Assay Time:

1.5H

Sample Type:

Serum, Plasma, Cell lysates, Cell culture supernates and Other biological fluids

Research:

Signal transduction;Infection immunity;Neuro science;Hormone metabolism;

Test Principle:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NE. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NE in the samples is then determined by comparing the OD of the samples to the standard curve.

Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD
5000	0.188	0.188	0.188
2500	0.326	0.327	0.327
1250	0.545	0.55	0.548
625	0.876	0.88	0.878
312.5	1.297	1.303	1.3
156.25	1.754	1.764	1.759
78.13	2.063	2.072	2.068
0	2.543	2.554	2.549

Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	88-102	81-94	86-97
1:2	Average (%)	96	84	92
1:4	Range (%)	96-107	81-94	80-94
1:4	Average (%)	106	90	89
1:8	Range (%)	89-103	80-90	90-99
1:8	Average (%)	93	84	95
1:16	Range (%)	80-95	83-95	87-103
1:16	Average (%)	80	85	100

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.