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AA(Arachidonic Acid) ELISA Kit
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Product No

FY-EU1191

Sample Type

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids

Assay Time

1.5H

Price($)

280 400

Size

48T 96T

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Product Details
Product Name in English:
AA(Arachidonic Acid) ELISA Kit
Species:
Universal
Alternative Name:
ARA
Assay Type:
Competitive
Standard:
200 μg/mL
Sensitivity:
0.94 μg/mL
Detection Range:
3.13-200 μg/mL
Assay Time:
1.5H
Sample Type:
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids
Research:
Signal transduction;Metabolic pathway;Endocrinology;Neuro science;Hormone metabolism;Bone metabolism;
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AA. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AA in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
200 0.174 0.175 0.175
100 0.31 0.311 0.311
50 0.525 0.527 0.526
25 0.859 0.862 0.861
12.5 1.287 1.292 1.29
6.25 1.679 1.683 1.681
3.13 2.055 2.075 2.065
0 2.495 2.499 2.497
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 92-105 89-98 92-103
Average (%) 97 94 94
1:4 Range (%) 92-101 89-98 86-97
Average (%) 100 94 87
1:8 Range (%) 80-94 81-94 83-96
Average (%) 85 91 85
1:16 Range (%) 86-101 96-105 91-103
Average (%) 89 104 99
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 90-105 97
EDTA plasma (n=8) 88-102 95
Cell culture media (n=8) 87-97 90
Assay Procedures
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