EasyPro Human VEGF-D(Vascular Endothelial Growth Factor D) ELISA Kit

Human

FIGF, VEGF-D, VEGFD, C-fos induced growth factor

O43915

Sandwich

4000pg/mL

37.5 pg/mL

62.5-4000pg/mL

1.5H

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Metabolic pathway;

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VEGF-D. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for VEGF-D are added to the micro ELISA plate wells. VEGF-D in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to VEGF-D. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of VEGF-D. You can calculate the concentration of VEGF-D in the samples by comparing the OD of the samples to the standard curve.