Product Name in English:
EasyPro Mouse PPAR-γ(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
Alternative Name:
glitazone receptor,NR1C3,PPARG,CIMT1,GLM1,PPARG1,PPARG2,PPARgamma,Nuclear receptor subfamily 1 group C member 3
Detection Range:
0.16-10ng/mL
Sample Type:
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uL
Research:
Signal Transduction; Cancer
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PPAR-γ. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for PPAR-γ are added to the micro ELISA plate wells. PPAR-γ in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to PPAR-γ. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PPAR-γ. You can calculate the concentration of PPAR-γ in the samples by comparing the OD of the samples to the standard curve.