Product No
FY-EH4594Sample Type
serum, plasma, Tissue homogenate and Other biological samples , Sample volume: 100μLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
20 | 3.171 | 3.194 | 3.183 | 3.147 |
10 | 2.196 | 2.218 | 2.207 | 2.172 |
5 | 1.323 | 1.335 | 1.329 | 1.299 |
2.5 | 0.736 | 0.737 | 0.737 | 0.712 |
1.25 | 0.382 | 0.383 | 0.383 | 0.358 |
0.63 | 0.202 | 0.203 | 0.203 | 0.178 |
0.32 | 0.091 | 0.092 | 0.092 | 0.067 |
0 | 0.024 | 0.024 | 0.024 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 90-105 | 97 |
EDTA plasma (n=8) | 97-105 | 101 |
Cell culture media (n=8) | 95-107 | 101 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 95-102 | 86-95 | 85-96 |
Average (%) | 100 | 91 | 94 | |
1:4 | Range (%) | 98-106 | 86-95 | 92-102 |
Average (%) | 103 | 87 | 93 | |
1:8 | Range (%) | 87-98 | 93-103 | 81-93 |
Average (%) | 96 | 97 | 84 | |
1:16 | Range (%) | 91-102 | 87-98 | 86-98 |
Average (%) | 99 | 89 | 95 |