Polyclonal antibodies

Polyclonal antibodies (pAbs) are antibodies with secretion by different B cell lineages within the body. These antibodies are a collection of immunoglobulin molecules, which can react against a specific antigen and identify a different epitope. The polyclonal antibody is a collection of anitbodies from different B cells, it can recognize multiple epitopes on the same antigen.

Production of Polyclonal Antibodies

The general production procedures of polyclonal antibodies are:

  1. Antigen preparation
  2. Adjuvant selection and preparation
  3. Animal selection
  4. Injection process
  5. Blood serum extraction

Advantage of Polyclonal Antibodies

  1. Robust target signal for even low expression protein
  2. Cost effective production with low cost and short production time
  3. More tolerant to antigen alternation(polymorphism, denature, heterogeneity of glycosylation)
  4. Detection across a wide range of species
  5. Applications for non-characterized antigens

Disadvantage of Polyclonal Antibodies

  1. High variability in batch to batch production
  2. Require higher background in certain applications
  3. Potential distraction and disruption in applications
  4. Significant lower lever of concentration comparing to monoclonal antibodies

Polyclonal antibody catalog