Tag antibodies
Tag antibodies are applied to detect and purify the multiple proteins with the same tag. Tag antibodies, also known as epitope tag antibodies, are highly specific monoclonal and polyclonal antibodies for the purification and detection of the tagged proteins. Epitope tags are small peptides with addition to proteins of interest in recombinant DNA techniques. These tags are perfect for the research of recombinant proteins, once protein-specific antibodies are not available or suitable for experimental design.
Feiyuebio provides a full range of tag antibodies to satisfy various research requirements, including: HA Tag antibodies, Myc Tag antibodies, DYKDDDDK Tag antibodies, GFP Tag antibodies, and V5 Tag antibodies. All these tag antibodies are fully validated in different applications, like: ELISA, Western Blotting (WB), Flow Cytometry (FCM), Immunoprecipitation (IP) and Immunofluorescence (IF).
Epitope Tags
The epitope tag is a biological structure or sequence like a protein, peptide, small molecule or carbohydrate, it acts as an antigen to be recognized by the specific antibody. Epitope tagging is the technology to fuse epitopes to the recombinant protein by genetic engineering or chemical conjugation. The epitope tags are able to detect proteins once no antibody is available. This technology is normally applied to characterize new protein discoveries and low abundant proteins.
Epitope tags are useful tools for several immuno-assays, including western blot, immuno-precipitation, immuno-fluorescence. In addition, it combines with affinity chromatography to purify target proteins from complex samples.
Types of Epitope Tags
As different epitope tags are available, the tag choice is dependent on several factors.
- Fluorescent peptide: Fluorescent tags Green Fluorescent Protein (GFP), Allophycocyanin (APC) are used for cell tracking and cellular localization.
- Protein purification: MBP, His, V5 and Glutathione S-Transferase (GST) are mainly for protein purification.
- Protein analysis: C-my, Hemagluttinin (HA), and DYKDDDDK are used in pull-down/immuno-precipitation experiments for protein-protein interaction verification and identification.
Advantage of Tag Antibodies
As epitope tags are applied for different size proteins without damage to native structure and function. Tag antibodies have several advantages:
- Detection and affinity purify tagged molecules
- Highly specific antibodies including rabbit recombinant antibodies
- Simplify experimental design
- Available bulk quantities with low cost
- Available sizes for small scale testing
- Multiple conjugation options
